Rachel Green | Current Members | Lab Alumni | Lab Action Shots


Julie Brunelle, Research Technician
Previous Insitution: Alma College
E-Mail: jbrunelle at jhmi dot edu

After graduating from Alma College, I began working in the Green lab in June 2000 as a research technician. Bacterial translation has been my main research focus, although I continue to work on a variety of projects in the lab depending on where I'm most useful. Over the years, I have been able to offer technical support to members of the Green lab and have learned many different techniques.

Allen Buskirk, Research Associate
Previous Insitution: Brigham Young University
E-Mail: buskirk at jhmi dot edu

My lab studies ribosome stalling in bacteria. Using genetic selections and in vitro kinetics, we identified and characterized nascent peptides that interfere with their own translation. Through our work on stalling peptides, we became interested in EFP, an elongation factor that suppresses pausing at polyproline motifs, and in the enzymes that modify EFP. In addition, we continue to work on determining the mechanism of how stalled ribosomes are recognized and recycled in bacteria. In 2014 we moved from BYU to Hopkins in order to collaborate more extensively with the Green lab.

Elizabeth Rogers, Lab Manager
Previous Insitution: University of Maryland, Baltimore County
E-Mail: eroger11 at jhmi dot edu

As lab manager I am responsible for ordering supplies, equipment repair and I also prep a number of common enzymes/reagents used by the lab. I also help out on specific projects when needed.


Kazuki Saito, Postdoctoral Researcher
Previous Institution: University of Tokyo
E-Mail: ksaito4 at jhmi dot edu

Current focus of my research is ribosome rescue in both canonical and aberrant translations. Using as well as modifying ribosome profiling, I am pursuing to elucidate the molecular mechanisms and the endogenous regulations of those phenomena.

Colin Wu, Postdoctoral Researcher
Previous Institution: Academia Sinica
E-Mail: colinccwu at gmail.com

I am interested in identifying Dom34:Hbs1-associated extra-ribosomal factors that are involved in no-go decay (NGD) and nonstop decay (NSD) pathways, using immunoprecipitation coupled with liquid chromatography tandem mass spectrometry (LC-MS/MS).

Boris Zinshteyn, Damon Runyon Foundation Postdoctoral Fellow
Previous Institution: Massachusetts Institute of Technology
E-Mail: borisz at jhmi dot edu

Nearly a third of heritable human disease-causing mutations result in the production of premature translation termination (or nonsense) codons. These premature termination events often result in the degradation of the encoding messenger RNA, preventing the accumulation of potentially harmful truncated proteins, through a process caused nonsense-mediated mRNA decay. I aim to determine the fundamental mechanism by which premature termination codons are differentiated from normal termination codons, independent of mRNA splicing.

Daniel Goldman, Damon Runyon Foundation Postdoctoral Fellow
Previous Institution: University of California - Berkeley
E-Mail: goldman at jhmi dot edu

Protein synthesis is regulated by messenger RNA (mRNA) processing events that occur before the first ribosome translates the mRNA. I am interested in how the "history" of an mRNA is communicated to the translation machinery and ultimately dictates the level of protein synthesis. In particular, I am studying the mechanism of enhanced translation conferred by mRNA splicing.

Graduate Students

Karole D'Orazio, Graduate Student, BCMB
Previous Institution: Stony Brook University
E-Mail: kdorazi1 at jhmi dot edu

I am interested in studying different factors that alter the activity of the ribosome. Specifically, I am looking at translation on poly(A) sequences and the interaction between the ribosome and the translation repressor, Dhh1.

Fuad Mohammad, Graduate Student, BCMB
Previous Institution: The Ohio State University
E-Mail: fmohamm6 at jhmi dot edu

I’m interested in studying the role of protein synthesis and degradation within the nervous system. Using molecular biology techniques and ribosome profiling, I wish to understand how depolarization can modulate translational control within neurons, as well as characterize rapid protein turnover by the proteasome.

Laura Nevin, Graduate Student, PMB
Previous Institution: University of Maryland Baltimore County
E-Mail: lnevin1 at jhu dot edu

I am interested in further characterizing the mechanisms of eukaryotic translation termination and ribosome recycling through the use of single molecule fluorescence studies. This work is performed using objective-based total internal reflection microscopy.

Aditya Radhakrishnan, Graduate Student, PMB
Previous Institution: Washington University in St. Louis
E-Mail: radhakrishnan at jhu dot edu

I'm interested in understanding the functional role of the highly conserved DEAD-box containing protein Dhh1, particularly rationalizing the diversity of observed phenotypes including decapping activation, translation repression, and its role in processing body dynamics. I'm currently using tethering assays to track interactions of ribosomes with mRNA that are mediated by Dhh1.

Anthony Schuller, Graduate Student, BCMB
Previous Institution: University of Pennsylvania
E-Mail: aschull2 at jhmi dot edu

I'm interested in studying the mechanism of nonsense-mediated decay (NMD) and specifically the function and interaction of Upf1 on the ribosome. I am currently using the in vitro yeast system to test how Upf1 and its associated factors (Upf2, Upf3, etc.) engage the ribosome to activate NMD.

Jamie Wangen, Graduate Student, BCMB
Previous Institution: St. Olaf College
E-Mail: jwangen1 at jhmi dot edu

I am interested in studying the role of translational regulation in the differentiation of hematopoietic cells, particularly of the erythroid lineage, using high-throughput approaches including ribosome profiling. Additionally, I hope to elucidate the particular roles of several ribosomal proteins governing this process.