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HOMEWOOD CAMPUS IMAGING, MICROSCOPY, and MICROANALYSIS

Regenova 3D Bioprinting Core

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IIC WIKI

Johns Hopkins University • Integrated Imaging Center • 3400 N. Charles Street • Dunning Hall • Baltimore, MD 21218 • Phones • Office: (410) 516-3321 • Laboratory: (410) 516-8042 • FAX: (410) 516-5213

The IIC has an FEI F200C Talos 200keV FEG transmission electron microscope (TEM) equipped with two 4K CMOS cameras, a Falcon 2 direct electron detecting camera; and a Ceta scintillated 4K CMOS camera.  


The FEI Talos™ transmission electron microscope was acquired via an NIH S10 grant in 2015. It is a very versatile system on which one can carryout the most routine, conventional TEM imaging; as well as the most advanced cryoTEM, 3D single particle reconstruction. It is ideal for routiine characterization of biological and nanobiomaterial samples for cell biology, structural biology, and nanobiotechnology research.


The Talos is also quite capable of high resolution imaging of materials; as well as electron diffraction. It is equipped with FEI's unique  constant-power, C-TWIN lens which ensures excellent optical performance with a perfect balance of contrast and resolution coupled with high tilt angles of +/- 85°.


It is housed in room 45 of the LEVI Building, part of the Mudd Hall complex; and is complemented by an FEI Vitrobot, a vitrification robot; and a Gatan 655 pumping station with two Gatan 626/70° cryo-transfer holders.


Training for the Talos can be arranged by contacting IIC Director, Michael McCaffery at <jmccaffery@jhu.edu>; or calling (410) 516-3321. As a general rule, a user will have first been trained on the Tecnai 12 G2 Spirit 120keV TEM located in Dunning Hall prior to using the Talos,