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HOMEWOOD CAMPUS IMAGING, MICROSCOPY, and MICROANALYSIS

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INSTRUMENTATION & EQUIPMENT


The Integrated Imaging Center has the necessary instrumentation (hardware and software) required for most basic and advanced LM/EM techniques. These include:


ELECTRON MICROSCOPES (and ancillary support equipment)

• Philips CM 300 FEG TEM equipped withGatan Orius SC200 and Gatan 200 EELS detector (acquired with equal funds from KECK foundation and NSF MRI # 9512438; Hemker and Veblen PIs.)

FEI Talos 200SC FEG TEM equipped with CETA and Falcon II Direct Detection CMOS cameras. (NIH SIG award # 1S10RR023454-01, McCaffery PI)

FEI Tecnai 12 TWIN TEM equipped with 16 bit 2K x2K FEI Eagle bottom mount camera; and SIS Megaview III wide-angle camera (acquired with funds from NSF DBI award # 0099706, McCaffery PI; and NIH SIG award # 1S10OD012342-01A1, McCaffery PI)

Philips EM 420 TEM equipped with SIS Megaview III CCD digital camera; and one with Oxford EDS dector. (acquired in part with funds from NIH R01GM060979-06, Wendland PI)

FEI Quanta 200 Environmental SEM (acquired with funds from HHMI undergraduate education grant)

JEOL 8600 SEM Microprobe;

FEI VITROBOT vitrification robot (acquired jointly with funds contributed by WSE and KSAS)

TOUSIMIS 795 critical point dryer;

Anatech Hummer 6.02 Sputter Coater;

Denton 502A High Vacuum Evaporator;

2 LEICA Ultracut UCT Ultramicrotomes/FCS Cryostages(acquired in part with funds from NSF DBI award # 0099706, McCaffery PI)


FLUORESCENCE MICROSCOPES (and related equipment):

Zeiss LSM 510 META Confocal with motorized stages and 405, 488, 543 and 633nm lasers (acquired with funds from NIH SIG award #1S10RR019409-01, McCaffery PI)

In Vivo Scientific cage incubators (acquired in part with funds from NIH SIG award #1S10RR019409-01, and #1S10RR25505-01; McCaffery PI)

Two Zeiss LSM 700 confocals with Pecon cage incubators for live imaging. Lasers include 405, 488, 555, and 633 nm.

Zeiss LSM 780 confocal microscope with Pecon cage incubator;

Zeiss AxioObserver Yokogawa CSU-X1 spinning disk confocal equipped with dual Evolve EMCCDs, 405/488/561and 633nm lasers, FRAP module, and stage incubator for live imaging. (acquired in part with NIH S10 award #1S10OD010712-01A1, PI McCaffery)

Zeiss LSM 510 Multiphoton and Confocal equipped with Confocor 3 Fluorescence Correlation Spectroscopy module an

Zeiss AxioObserver Epifluorescence SIM microscope equipped with Apotome 2 and Hamamatsu Orca Flash 4.0 CMOS camera. (acquired in part with NIH S10 award #1S10OD010712-01A1, PI McCaffery)

3i Marianas Live Cell Imaging Workstation with In Vivo Scientific cage incubator equipped with dual Cascade II 512 EM cameras for simultaneous 2-channel acquistion and TIRF (acquired in part with NIH S10 award #1S10OD010712-01A1, PI McCaffery)

Zeiss Axiovert 200 LM with In Vivo Scientific cage incubator equipped with excitation and emission filter wheels; and Cooke Sensicam camera; also equipped for microinjection and micromanipulation (acquired in part with funds from NIH SIG award #1S10RR019409-01, McCaffery PI)

Zeiss Axiovert 100 LM equipped with a CoolSnap digital monochrome camera; and and I-Pentamax 512 digital camera (acquired in part with funds from NIH SIG award #1S10RR019409-01, McCaffery PI; and NIH R01GM060979-06, Wendland PI)

Zeiss Axioplan II LM equipped with a Cooke Sensicam digital monochrome camera; and a Q-Imaging color camera (This system is excellent for polarized microscopy and color photomicrography.)

Zeiss AxioZoom V16 Fluorescence Stereoscope equipped with Axiocam MRm camera.

Bioptech FCS-2 Environmental Stage(acquired in part with funds from NIH SIG award #1S10RR019409-01, McCaffery PI)

Instec 0°-60°C stage, peltier modulated (acquired in part with funds from NIH SIG award #1S10RR019409-01, McCaffery PI)

Typhoon 9410 Variable Mode Imager capable of storage phosphor, chemiluminescence, and fluorescence (acquired with funds from NIH SIG award 1S10RR021023-01, McCaffery PI)


CELL SORTERS and FLOW CYTOMETERS:


Sony Biotechnology SH800 Cell Sorter (acquired with funds from NIH SIG award 1S10OD016230-01A1, McCaffery PI)

Sony Biotechnology SY3200 Cell Sorter (acquired with funds from NIH SIG award 1S10OD016230-01A1, McCaffery PI)

BD FACSCalibur Flow Cytometer;

BD FACSCanto High-throughput Flow Cytometer;


MISCELLANEOUS/OTHER EQUIPMENT:


Avestin EmulsiFlex C3 (acquired in part with funds from NIH R01GM060979-06, Wendland PI)

Eppendorf Microijector 5170/Micromanipulator 5242;

IICDMP server, 50 Tbytes expandable to 1PB (acquired in part with funds from NIH SIG awards 1S10RR021023-01, McCaffery PI; and 1S10RR019409-01, McCaffery PI )

IICXServer with 14.0 Tbytes (acquired in part with funds from NIH SIG #1S10RR25505-01); McCaffery PI)

Remote Linux, Mac, and PC image processing/analysis workstations;

Johns Hopkins University • Integrated Imaging Center • 3400 N. Charles Street • Dunning Hall • Baltimore, MD 21218 • Phones • Office: (410) 516-3321 • Laboratory: (410) 516-8042 • FAX: (410) 516-5213