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HOMEWOOD CAMPUS IMAGING, MICROSCOPY, and MICROANALYSIS

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SCHEDULER

IIC WIKI

       LIGHT MICROSCOPY                                                                                               ELECTRON MICROSCOPY


• Acid Phosphatase                                                                                                • Acid Phosphatase

• Antibody Purification                                                                                            • Akaline Phosphatase

• Eisen Buffer (PBS)                                                                                                • Whole Animal perfusion/fixation

• Formaldehyde fixation for immunofluorence                                                         • Au-A-PA Preparation

• Gelatin Coating of Slides                                                                                      • Colloidal Gold Preparation

• Glycogen Staining                                                                                                • Drosophila ImmunoEM

• IF on Cells with Biotin/Avidin                                                                                • Drosophila Morphology

• IF on semi-thin cryosections                                                                                • TEM on Cellular Fractions

• in-situ hybridization                                                                                              • Formaldehyde Fixation for ImmunoEM

• Immunoperoxidase on Cells                                                                                 • Formvar Grids

• Yeast Immunofluorescence                                                                                  • Immunodiffusion

• Gelatin Coated Coverslips                                                                                   • Immunogold labeling of ultrathin Cryosections   

                                                                                                                                • Kleinschmidt Technique

                                                                                                                                • Yeast KMnO4 fixation

                                                                                                                                • Yeast OTO fixation   

                                                                                                                                • LR White Processing

                                                                                                                                • Metal Shadowing

                                                                                                                                • Neutral Uranyl acetate

                                                                                                                                • Uranyl formate

                                                                                                                                • PLP Fixation (Nakane and McLean)

                                                                                                                                • Protein A Gold    

                                                                                                                                • Routine Morophology                                                                                              

Johns Hopkins University • Integrated Imaging Center • 3400 N. Charles Street • Dunning Hall • Baltimore, MD 21218 • Phones • Office: (410) 516-3321 • Laboratory: (410) 516-8042 • FAX: (410) 516-5213