HOMEWOOD CAMPUS IMAGING, MICROSCOPY, and MICROANALYSIS
TECHNIQUES and METHODOLOGIES
• Remote, web-based imaging is available for all LM/EM applications (via Microsoft NetMeeting).
• All types of conventional electron microscopy including routine plastic embedding prep.; negative staining; metal shadowing; "Kleinschmidt"(RNA/DNA prep).
• High Resolution analytical transmission electron microscopy with EELS (FEI CM 300kV with Gatan 2000) and EDS (with Philips EM 420 120kV and Oxford detector)
• All aspects of Immunocytochemistry including immunofluorescence; immunogold; and immunoperoxidase on pre- and post-embedded samples (i.e. cryosections and hydrophilic embedment).
• cryo-Transmission Electron Microscopy
• Contrast enhancement of LM specimens via DIC (Nomarski); phase; and digital techniques.
• Epifluorescence utilizing multiple excitation and/or emission wavelengths with high temporal resolution for ratio imaging and other applications.
• Ultra-fast simultaneous two-channel TIRF fluorescence acquisition with dual Cascade II 512 EM cameras.
• Ultra-low light level microscopy.
• Multi-probe detection utilizing deconvolution and confocal microscopy.
• 3-D and 4-D imaging.
• Numerous digital image enhancement/analysis techniques including point-spread deconvolution, 2-D Fourier filtering, motion analysis, sterometry, and ratio imaging.
• Cell ablation, and Fluorescence Recovery After Photobleaching (FRAP).
• Photoactivation of Intracellular Probes.
• Fluorescence Resonance Energy Transfer (FRET) acceptor photobleaching and sensitized emmission.
• Fluorescence Lifetime Imaging Microscopy (FLIM)...Time-resolved correlated fluorescence
• In vitro and in vivo Fluorescence Correlation Spectroscopy (FCS), with single molecule detection, auto-correlation, and cross-correlation.
• Multiphoton microscopy for deep-tissue imaging and FCS via Chameleon Ultra II Ti:Sapphire laser.
Johns Hopkins University • Integrated Imaging Center • 3400 N. Charles Street • Dunning Hall • Baltimore, MD 21218 • Phones • Office: (410) 516-3321 • Laboratory: (410) 516-8042 • FAX: (410) 516-5213