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HOMEWOOD CAMPUS IMAGING, MICROSCOPY, and MICROANALYSIS

Regenova 3D Bioprinting Core

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SCHEDULER

IIC WIKI


TECHNIQUES and METHODOLOGIES


Remote, web-based imaging is available for all LM/EM applications (via Microsoft NetMeeting).

All types of conventional electron microscopy including routine plastic embedding prep.; negative staining; metal shadowing; "Kleinschmidt"(RNA/DNA prep).

High Resolution analytical transmission electron microscopy with EELS (FEI CM 300kV with Gatan 2000) and EDS (with Philips EM 420 120kV and Oxford detector)

All aspects of Immunocytochemistry including immunofluorescence; immunogold; and immunoperoxidase on pre- and post-embedded samples (i.e. cryosections and hydrophilic embedment).

cryo-Transmission Electron Microscopy

Contrast enhancement of LM specimens via DIC (Nomarski); phase; and digital techniques.

Epifluorescence utilizing multiple excitation and/or emission wavelengths with high temporal resolution for ratio imaging and other applications.

Ultra-fast simultaneous two-channel TIRF fluorescence acquisition with dual Cascade II 512 EM cameras.

Ultra-low light level  microscopy.

Multi-probe detection utilizing deconvolution and confocal microscopy.

3-D and 4-D imaging.

• Numerous digital image enhancement/analysis techniques including point-spread deconvolution, 2-D Fourier filtering, motion analysis, sterometry, and ratio imaging.

Microspectrophotometry.

Cell ablation, and Fluorescence Recovery After Photobleaching (FRAP).

Photoactivation of Intracellular Probes.

Fluorescence Resonance Energy Transfer (FRET) acceptor photobleaching and sensitized emmission.

Fluorescence Lifetime Imaging Microscopy (FLIM)...Time-resolved correlated fluorescence

In vitro and in vivo Fluorescence Correlation Spectroscopy (FCS), with single molecule detection, auto-correlation, and cross-correlation.

Multiphoton microscopy for deep-tissue imaging and FCS via Chameleon Ultra II Ti:Sapphire laser.




















Johns Hopkins University • Integrated Imaging Center • 3400 N. Charles Street • Dunning Hall • Baltimore, MD 21218 • Phones • Office: (410) 516-3321 • Laboratory: (410) 516-8042 • FAX: (410) 516-5213