1968 Ph.D. (Pharmacology)  University of Southern California
2011 - present Research Professor, Department of Biomedical Engineering, Whiting School of Engineering, Johns Hopkins University
2007 - 2010 Research Professor, Department of Chemistry and Advanced Technology Laboratory, Whiting School of Engineering, Johns Hopkins University
2003 - Present Director Microscopy Center,
Montgomery County Campus, Johns Hopkins University
1998 -  2007 Research Professor, Department of Biology, Johns Hopkins University
1996 -1998 Professor of Cell Biology, Department of Cell Biology, Georgetown University
1986 - 1993 Member, Fidia-Georgeotwn Institute for the Neurosciences, Chief Lab of Ion and 2nd Messenger Imaging
1985 - 2003 Founder and Chief Scientific Officer, Atto Instruments, Inc. (now Atto Bioscience, Inc.). Atto Bioscience, Inc., acquired by BD (Becton, Dickinson and Company) on July 1, 2004.
1981 - 1998 Professor of Biochemistry and Molecular Biology, Department of Biochemistry and Molecular Biology, Georgetown University
1976 - 1981 Director Core Program and Associate Director, University of Virginia Diabetes Research Center
1972 -1981 Associate Professor and Professor of Pharmacology, Department of Pharmacology, University of Virginia School of Medicine
1968 -1972 Assistant Professor of Medicine and Biochemistry, University of Southern California School of Medicine

Scientific and Professional Societies
  • Fellow, Optical Society of America
  • Member, American Society for Biochemistry and Molecular Biology
  • Member, Society of Neuroscience
  • Member, American Association for the Advancement of Science

Research Interests
  • Mechanism of receptor independent hormone desensitization
  • Mechanisms of resistance to chemotherapeutic agents
  • Optical methods in microscopy
Publications

Brooker, G., Thomas, L. J. and Appleman, M. Michael:  The Assay of Adenosine 3'5' Cyclic Monophosphate and Guanosine 3'5' Cyclic Monophosphate in Biological Materials by Enzymatic Radioisotopic Displacement.  Biochemistry 7, 4177, 1968.

Brooker, G., and Appleman, M. Michael:  The Theoretical Basis for the Measurement of Compounds by Enzymatic Radioisotopic Displacement.  Biochemistry 7, 4182, 1968.

Brooker, G. and Thomas, L. J.:  Phosphatase and Ouabain Sensitive ATPase of the Perfused Frog Heart.  Molecular Pharmacology 7, 199, 1971.

Brooker, G.:  Determination of Picomole Amounts of Enzymatically Formed Adenosine 3'5' Cyclic Monophosphate by High Pressure Anion Exchange Chromatography.  Analytical Chemistry 42, 1108, 1970.

Brooker, G.:  Measurement of Cyclic AMP by High Pressure Anion Exchange Chromatography.  In Nucleic Acid Constituents by Liquid Chromatography, by Burtis and Gere, Varian Aerograph, 1970, Walnut Creek, California.

Bell, J., Brooker, G. and Harding, B. W.:  ACTH Activation of Glycolysis in the Rat Adrenal Gland.  Biochem. Biophys. Res. Comm. 41, 938, 1970.

Brooker, G. and Fichman, M.:  Chlorpropamide and Tolbutamide Inhibition of Cyclic AMP Phosphodiesterase.  Biochem. Biophys. Res. Comm. 42, 824, 1971.

Brooker, G. and Jelliffe, R. W.:  Serum Cardiac Glycoside Assay Based Upon Displacement of 3H Ouabain from Na-K ATPase.  Circulation 45, 20, 1972.

Brooker, G.:  The Effect of Temperature Control on the Stability and Sensitivity of a High Pressure Liquid Chromatography Ultraviolet Flow Cell Detector.  Analytical Chemistry 43, 1095, 1971.

Brooker, G.:  High Pressure Anion Exchange Chromatographic Measurement of Cyclic 3',5' Adenosine Monophosphate and 14C Cyclic AMP Specific Activity in Myocardium Prelabeled with 14C Adenosine.  Journal of Biological Chemistry 246, 7810, 1971.

Brooker, G.:  High Pressure Anion Exchange Chromatography and Enzymatic Isotope Displacement.  Assays for Cyclic AMP and Cyclic GMP.  In Advances in Cyclic Nucleotide Research 2, 111, 1972.  Greengard, Robison and Paoletti, editors, Raven Press, New York.

Brooker, G. and Jelliffe, R. W.:  Enzymatic Isotope Displacement Analysis of Cardiac Glycosides.  In Modern Methods of Steroid Analysis.  Erich Heftmann, editor, 437-449, 1973.  Academic Press, New York.

Jelliffe, R. W. and Brooker, G.:  A Nomogram for Digoxin Therapy.  American Journal of Medicine 57, 63, 1974.

Brooker, G.:  High Pressure Liquid Chromatography for the Analysis of Cyclic Nucleotides.  In Methods in Molecular Biology   3, 81, 1972.  M. Chasin, editor.  Marcel Dekker, New York.

Fichman, M. P., Littenberg, G., Brooker, G. and Horton, R.:  The Effect of Prostaglandin A (PGA1) on Renal and Adrenal Function in Man.  Circulation Research, Supplement II to Vol.  30-31 , 11-19, 1972.

Brooker, G.:  Newer Developments in the Determination of Cyclic AMP and Other Cyclic Nucleotides, Adenylate Cyclase and Phosphodiesterase.  In Methods of Biochemical Analysis 22, 95-121.  David Glick, editor.  John Wiley and Sons.

Fichman, M. P. and Brooker, G.:  Deficient Renal Cyclic AMP Production in Nephrogenic Diabetes Insipidus.  Journal of Clinical Endocrinology and Metabolism 35, 35, 1972.

Brooker, G.:  Change in Myocardial Adenosine 3',5' Cyclic Monophosphate During the Contraction Cycle.  In Myocardial Metabolism 3, 207-212, 1973.  N. S. Dhalla, editor.  (Vol. 3 in  Recent Advances in Cardiac Structure and Metabolism, E. Bajusz and G. Rona, editors.)  University Park Press, Baltimore.

Brooker, G.:  Separation, Purification and Analysis of Cyclic Nucleotides by High Pressure Ion Exchange Chromatography.  In Methods in Enzymology, B. W. O'Malley and J. G. Hardman, volume editors, Vol. XXXVIII, pp. 20-26, 1974.  Academic Press.

Brooker, G.:  Frog Ventricular Muscle Suitable for Use in Measuring Rapid Metabolic Changes During the Contractile Cycle.  In Methods in Enzymology, B. W. O'Malley and J. G. Hardman, volume editors, Vol. XXXIX, pp. 60-63, 1975.  Academic Press.

Thompson, J. W., Brooker, G. and Appleman, M. M.:  Assay of Cyclic Nucleotide Phosphodiesterases with Radioactive Substrates.  In Methods in Enzymology, B. W. O'Malley and J. G. Hardman, volume editors, Vol. XXXVIII, pp. 205-212, 1974.  Academic Press.

de Vellis, J. and Brooker, G.:  Induction of Enzymes by Glucocorticoids and Catecholamines in a Rat Glial Cell Line.  In Tissue Culture of the Nervous System, G. Sato, editor, p. 231-245, Plenum Press, New York, 1973.

Brooker, G.:  Oscillation of Cyclic Adenosine Monophosphate Concentration During the Myocardial Contraction Cycle.  Science 182, 933, 1973.

de Vellis, J. and Brooker, G.:  Reversal of Catecholamine Refractoriness by Inhibitors of RNA and Protein Synthesis.  Science 186, 1221, 1974.

Brooker, G.:  Implications of Cyclic Nucleotide Oscillations During the Myocardial Contraction Cycle.  In Advances in Cyclic Nucleotide Research, 5, 435-452, 1975, G. Drummond, P. Greengard, and G. Robison, editors.  Raven Press, New York.

Nakai, C. and Brooker, G.:  Assay for Adenylate Cyclase and Cyclic Nucleotide Phosphodiesterases and the Preparation of High Specific Activity 32P-Labeled Substrates.  Biochimica et Biophysica Acta 391, 222, 1975.

Schneider, J. A., Brooker, G. and Sperelakis, N.:  Papaverine Blockade of an Inward Slow Ca++ Current of Guinea Pig Heart.   Journal of Molecular and Cellular Cardiology 7, 867, 1975.

Hsu, C.-Y., Brooker, G., Peach, M. J. and Westfall, T. C.:  Inhibition of Catecholamine Release by Tolbutamide and Other Sulfonylureas.  Science 187, 1086, 1975.

Brooker, G.: Review of:  Cyclic AMP, Cell Growth, and the Immune Response, by W. Braun, L. Lichtenstein, and C. Parker.  In Science 186, 1029, 1974.

Harper, J. F. and Brooker, G.:  Femtomole Sensitive Radioimmunoassay for Cyclic AMP and Cyclic GMP after 2'0 Acetylation by Acetic Anhydride in Aqueous Solution.  Journal of Cyclic Nucleotide Research 1, 207, 1975.

Terasaki, W. L. and Brooker, G.:  Automated Method for the Quantitation of Orthophosphate.  Analytical Biochemistry 75, 447, 1976.

Brooker, G., Terasaki, W. L. and Price, M. G.:  Gammaflow:  The Completely Automated Radioimmunoassay.  Science 194, 270, 1976.

Larner, J., Takeda, Y., Brewer, H. B., Huang, L. C., Hazen, R., Brooker, G., Murad, F. and Roach, P.:  Studies on Glycogen Synthase and Its Control by Hormones.  In Metabolic Interconversion of Enzymes 1975, edited by S. Shaltiel, Springer-Verlag, Berlin, 1976, pp. 71-85.

Terasaki, W. L. and Brooker, G.:  Cardiac Adenosine 3':5'-Monophosphate:  Free and Bound Forms in the Intact Rat Atrium.  Journal of Biological Chemistry 252, 1041, 1977.

Hsu, C. Y., Leighton, H. J., Westfall, T. C. and Brooker, G.:  Enhancement of Methacholine-Stimulated Guanosine 3':5'-Cyclic GMP Monophosphate Formation in Supersensitive Guinea Pig Vas Deferentia.  Journal of Cyclic Nucleotide Research 2, 359, 1976.

Harper, J. F. and Brooker, G.:  Refractoriness to Muscarinic and Adrenergic Agonists in the Rat Parotid:  Responses of Adenosine and Guanosine Cyclic 3',5'-Monophosphates.  Molecular Pharmacology 13, 1048, 1977.

Linden, J. and Brooker, G.:  Surfactants Alter the Oxygen Tension of Buffers:  An Artifactual Component of the Inotropic Response to Sulfonylureas in Rat Atria.  Federation Proceedings 36, 2730, 1977.

Brooker, G.:  Dissociation of Cyclic GMP from the Negative Inotropic Action of Carbachol in Guinea Pig Atria.  Journal of Cyclic Nucleotide Research 3, 407, 1977.

Vogel, S., Sperelakis, N., Josephson, I. and Brooker, G.:  Fluoride Stimulation of Slow Ca2+ Current in Cardiac Muscle.  Journal of Molecular and Cellular Cardiology 9, 461-475, 1977.

Terasaki, W. L., Brooker, G., de Vellis, J., Hsu, C. Y., Inglish, D. and Moylan, R. D.:  Involvement of Cyclic AMP and Protein Synthesis in Catecholamine Refractoriness.  Advances in Cyclic Nucleotide Research, Vol. 9, 33, 1978.

Ackerly, J., Blumberg, A., Brooker, G., and Peach, M.:  Angiotensin II on the Release of D H and Atrial Cyclic AMP Concentrations.  American Journal of Physiology 235, 281, 1978.

Linden, J. and Brooker, G.:  Sodium Requirement for the Positive Inotropic Action of Isoproterenol on Guinea Pig Atria.  Science 199, 539, 1978.

Linden, J. and Brooker, G.:  The Positive Inotropic Action of Sulfonylureas:  A Mechanism Independent of Cyclic Adenosine 3':5'-Monophosphate.  Diabetes 27, 694, 1978.

Nickols, G. A. and Brooker, G.:  Temperature Sensitivity of Cyclic AMP Production and Catecholamine-Induced Refractoriness in a Rat Astrocytoma Cell Line.  Proceedings of the National Academy of Science 75, 5520, 1978.

Harper, J. F. and Brooker, G.:  Amylase Secretion from the Rat Parotid:  Refractoriness to Muscarinic and Adrenergic Agonists.  Molecular Pharmacology 14, 1031, 1978.

Terasaki, W. L. and Brooker, G.:  [125I]-Iodohydroxybenzylpindolol Binding Sites on Intact Rat Glioma Cells.  Journal of Biological Chemistry 253, 5418, 1978.

Larner, J., Roach, P. J., Huang, L. C., Brooker, G., Murad, F. and Hazen, R.:  Hormonal Control of Glycogen Metabolism.  In Hormones and Energy Metabolism (1979), edited by D. M. Klachko, R. R. Anderson, and M. Heimberg, Plenum Pub., New York, pp. 103-123, 1979.

Brooker, G., Harper, J. F., Terasaki, W. L. and Moylan, R. D.:  Radioimmunoassay of Cyclic AMP and Cyclic GMP.  Advances in Cyclic Nucleotide Research 10, 1, 1979.

Linden, J. and Brooker, G.:  The Questionable Role of Cyclic Guanosine 3':5'-Monophosphate in Heart.  Biochemical Pharmacology 28, 3351, 1979.

Gibbs, J. B., Hsu, C. Y., Terasaki, W. L. and Brooker, G.:  Calcium and Microtubule Dependence for Increased Ornithine Decarboxylase Activity Stimulated by Beta-Adrenergic Agonists, Dibutyryl Cyclic AMP, or Serum in a Rat Astrocytoma Cell Line.  Proceedings of the National Academy of Science 77, 995, 1980.

Terasaki, W. L., Linden, J. and Brooker, G.:  The Quantitative Relationship Between Beta-Adrenergic Receptor Number and Physiologic Responses as Studied with the Long Lasting Beta-Adrenergic Antagonist, Aminobenzylpropranolol.  Proceedings of the National Academy of Science 76, 6401, 1979.

Nickols, G. A. and Brooker, G.:  Potentiation of Cholera Toxin-Stimulated Cyclic AMP Production on Cultured Cells by Inhibitors of RNA and Protein Synthesis.  Journal of Biological Chemistry 255, 23, 1980.

Nickols, G. A. and Brooker, G.:  Induction of Refractoriness to Isoproterenol by Prior Treatment of C6-2B Rat Astrocytoma Cells with Cholera Toxin.  Journal of Cyclic Nucleotide Research 5, 435, 1979.

Harper, J. F. and Brooker, G.:  Alcohol Potentiation of Isoproterenol Stimulated Cyclic AMP Accumulation in Rat Parotid.  Journal of Cyclic Nucleotide Research 6, 51, 1980.

Linden, J. and Brooker, G.:  The Influence of Resting Membrane Potential on the Effect of Verapamil on Atria.  Journal of Molecular and Cellular Cardiology 12, 325, 1980.

Linden, J. and Brooker, G.:  Properties of Cardiac Contractions in Zero Sodium Solutions: Intracellular Free Calcium Controls Slow Channel Conductance.  Journal of Molecular and Cellular Cardiology 12, 457, 1980.

Linden, J. and Brooker, G.:  Sodium Requirement for the Contractile Effect of Ouabain on Isolated Guinea Pig Atria.  Circulation Research 46, 553, 1980.

Brooker, G. and Murad, F.:  Automated "Gamma-Flo" Radioimmunoassay of Urinary Cyclic AMP.  Clinical Chemistry 26, 1738-1740, 1980.

Barovsky, K. and Brooker, G.:  (-)-[125I]-Iodopindolol, A New Highly Selective Radioiodinated Beta-Adrenergic Receptor Antagonist:  Measurement of Beta-Receptors on Intact Rat Astrocytoma Cells.  Journal of Cyclic Nucleotide Research 6, 297-307, 1980.

Moylan, R. D., and Brooker, G.:  Cycloheximide Potentiation of PGE1 and Cholera Toxin Stimulated Cyclic AMP Accumulation in NG108-CC15 Neuroblastoma-Glioma Hybrid Cells.  Journal of Biological Chemistry 256, 6573-6576, 1981.

Lemke, T. L., Cramer, M. B., Adamski, S. W., Pedone, C. A. and Brooker, G.:  Beta-adrenergic Blocking Agents.  Alpha- and Gamma-Methyl(aryloxy)propanolamines.  Journal of Medicinal Chemistry 24, 1211, 1981.

Brooker, G., Fleming, J. W., and Ross, E. M.:  Radioimmunoassay of Digitonin.  Biochimica et Biophysica Acta 685, 39-43, 1982.

Brooker, G.:  Radioimmunoassay Techniques for Cyclic Nucleotides.  In Handbook of Experimental Pharmacology, Vol. 58/I, edited by J. A. Nathanson and J. W. Kebabian.  Springer-Verlag, 1982.

Moylan, R. D., Barovsky, K., and Brooker, G.:  N6,O2'-Dibutyryl Cyclic AMP and Cholera Toxin-induced Beta-adrenergic Receptor Loss in Cultured Cells.  Journal of Biological Chemistry 257, 4947-4950, 1982.

Linden, J. and Brooker, G.:  Evidence for Persistent Activation of Cardiac Slow Channels in Low-calcium Solutions.  American Journal of Physiology 242, H827-H833, 1982.

Brooker, G., Pedone, C., and Barovsky, K.:  Selective Reduction of Forskolin-Stimulated Cyclic AMP Accumulation by Inhibitors of Protein Synthesis.  Science 220, 1169-1170, 1983.

Gibbs, J. B. and Brooker, G.:  Calcium Dependence for Increased Antizyme Inhibitory Activity of Ornithine Decarboxylase in Rat Glioma Cells.  Biochimica et Biophysica Acta 801, 99-105, l984.

Gibbs, J. B. and Brooker, G.:  Dissociation of RNA Synthesis from the Calcium Requirement for Serum-increased Ornithine Decarboxylase Activity in Rat Glioma Cells.  Biochimica et Biophysica Acta 801, 87-98, l984.

Barovsky, K., Pedone, C., and Brooker, G.:  Forskolin-Stimulated Cyclic AMP Accumulation Mediates Protein Synthesis-Dependent Refractoriness in C6-2B Rat Glioma Cells.  Journal of Cyclic Nucleotide and Protein Phosphorylation Research 9, 181-189, 1983.

Barovsky, K., Pedone, C., and Brooker, G.:  Distinct Mechanisms of Forskolin-Stimulated Cyclic AMP Accumulation and Forskolin-Potentiated Hormone Responses in C6-2B Cells.  Molecular Pharmacology, 25, 256-260, l984.

Barovsky, K., and Brooker, G.: Forskolin Potentiation of Cholera-Toxin Stimulated Cyclic AMP Accumulation in Intact C6-2B Cells: Evidence for Enhanced Gs-C Coupling.  Molecular Pharmacology, 28, 502-507, 1985.

Brooker, G. and Pedone, C.: Maintenance of Whole Cell Isoproterenol and Forskolin Responsiveness in Adenylate Cyclase of Permeabilized Cells.  Journal of Cyclic Nucleotide and Protein Phosphorylation Research, 11, 113-122, 1986.

DeBernardi, M. and Brooker, G.:  Diphtheria toxin prevents catecholamine desensitization of A431 Human epidermoid carcinoma cells.  Proceedings of the. National Academy of Sciences USA, 84, 2246-2250, 1987.

Smith, A. A., Brooker, T. and Brooker, G.  Expression of rat mRNA coding for hormone stimulated adenylate cyclase in Xenopus oocytes.  FASEB Journal 1: 380-387. 1987.

Brooker, G.: Improved Radioimmunoassay of Cyclic Nucleotides, In Hormone Action: Cyclic Nucleotide Action and DegradationMethods in Enzymology, 159: 45-50, 1988.

DeBernardi, M. and Brooker, G.  Diphtheria toxin prevents catecholamine desensitization of A431 human epidermoid carcinoma cells. Proc Natl Acad Sci USA. 84(8):2246-50. 1987

Dal Toso, R., De Bernardi, M.A., Brooker, G., Costa, E., Mocchetti, I. Beta adrenergic and prostaglandin receptor activation increases nerve growth factor mRNA content in C6-2B rat astrocytoma cells. J Pharmacol Exp Ther. 246(3):1190-3. 1988

Danysz, W., Wroblewski, J.T., Brooker, G., Costa, E., Modulation of glutamate receptors by phencyclidine and glycine in the rat cerebellum: cGMP increase in vivo. Brain Res. 479:270-6. 1989

Mocchetti, I., De Bernardi, M.A., Szekely, A.M., Alho, H., Brooker, G., Costa, E., Regulation of nerve growth factor biosynthesis by beta-adrenergic receptor activation in astrocytoma cells: a potential role of c-Fos protein. Proc Natl Acad Sci U S A. 86:3891-5. 1989

Brooker, G., Seki, T., Croll, D., Wahlestedt, C.  Calcium wave evoked by activation of endogenous or exogenously expressed receptors in Xenopus oocytes. Proc Natl Acad Sci U S A. 87:2813-7. 1990

De Erausquin, G.A., Manev, H., Guidotti, A., Costa, E., Brooker, G., Gangliosides normalize distorted single-cell intracellular free Ca2 dynamics after toxic doses of glutamate in cerebellar granule cells  Proc Natl Acad Sci U S A. 87(20):8017-21. 1990

Guidotti, A., Manev, H., Favaron, M., Brooker, G., Costa, E. Protection by natural and semisynthetic gangliosides from Ca2-dependent neurotoxicity caused by excitatory amino acid (EAA) neurotransmitters. Adv Exp Med Biol. 268:135-44. 1990

Manev, H., Favaron, M., Bertolino, M., Brooker, G., Guidotti, A. and Costa, E.: Importance of Sustained Protein Kinase C Translocation and Destabilization of Ca2+ Homeostasis in Glutamate-Induced Neuronal Death. In: Neurotoxicity of Excitatory Amino Acids. A. Guidotti (ed). Raven Press, New York pp. 63-78, 1990.

Manev, H., Favaron, M., DeErausquin, G., Guidotti, A., Brooker, G. and Costa, E.: Destabilization of Ionized Ca2+  Homeostasis in Excitatory Amino Acid Neurotoxicity: Antagonism by Glycosphingolipids. Cell Biol. Int. Reports, 14: 3-14, 1990.

Yakovlev, A.G., De Bernardi, M.A., Fabrazzo, M., Brooker, G., Costa, E., Mocchetti, I.  Regulation of nerve growth factor receptor mRNA content by dexamethasone: in vitro and in vivo studies.  Neurosci Lett. 116:216-20. 1990

Zhang, H., Desai, N.N., Olivera, A. Seki, T., Brooker, G., Spiegel, S., Sphingosine-1-phosphate, a novel lipid, involved in cellular proliferation. J Cell Biol. 114(1):155-67. 1991.

DeBernardi,M.A., Seki, T. and Brooker G.,  Inhibition of cAMP accumulation by intracellular calcium mobilization in C6-2B cells stably transfected with substance K receptor cDNA. Proc Natl Acad Sci U S A. 88(20):9257-61. 1991

DeBernardi, M.A., and Brooker, G., 2-Aminopurine inhibits RNA and protein synthesis and reduces catecholamine desensitization in C6-2B rat glioma cells. FEBS Letters 296, 112-116, 1992.

De Erausquin,G., Brooker, G., Costa, E. and  Wojcik, W.J.,Stimulation of high affinity gamma-aminobutyric acid receptors potentiates the depolarization-induced increase of intraneuronal ionized calcium content in cerebellar granule neurons. Mol Pharmacol. 42(3):407-414 1992.

De Erausquin, G., Brooker, G., Hanbauer, I., K(+)-evoked dopamine release depends on a cytosolic Ca2+ pool regulated by N-type Ca2+ channels.  Neurosci Lett. 145(2):121-5 1992.

Debernardi, M.A., Munshi, R., and Brooker, G., Ca-2+ Inhibition of Beta-Adrenergic Receptor-Stimulated and Forskolin-Stimulated cAMP Accumulation in C6-2B Rat Glioma Cells is Independent of Protein Kinase-C. Molec Pharm. 43(3):451-458, 1993.

Cooper D.M.F., and Brooker, G., Ca-2+-Inhibited Adenylyl Cyclase in Cardiac Tissue, Trends in Pharmacological Sciences 14(2):34-36 1993.

DeBernardi, M.A., Munshi, R., Yoshimura, M., Cooper, D.M.F., and Brooker, G.  Predominant expression of type-VI adenylate cyclase in C6-2B rat glioma cells may account for inhibition of cyclic AMP accumulation by calcium.  Biochemical J. 293, 325-328, 1993.

Desai, N., Carlson, R. O., Mattie, M.E., Olivera, A., Buckley, N. E., Seki, T., Brooker, G., and Spiegel, S.  Signaling Pathways for Sphingosylphosphorylcholine-mediated Mitogenesis in Swiss 3T3 Fibroblasts. J Cell Biol. 121, 1385-1395, 1993.

Munshi, R., De Bernardi, M.A., and Brooker, G.  P2U receptors on C6-2B rat glioma cells:  Sequential changes in the levels of inositol phosphates, intracellular calcium and cAMP are modulated by protein kinase C.  Mol. Pharmacol. 44, 1185-1191, 1993.

Mattie, M., Brooker, G., and Spiegel, S. Sphingosine-1-phosphate, a putative second messenger, mobilizes calcium from internal stores via an inositol trisphosphate-independent pathway.  J. Biol. Chem. 269, 3181-3188, 1994.

Kiedrowski, L., Brooker, G., Costa, E., and Wroblewski, J.T.  Glutamate Impairs Neuronal Calcium Extrusion while Reducing Sodium Gradient. Neuron 12, 295-300, 1994.

DeErausquin, G., Brooker, G., Costa, E., and Hanbauer, I.  Persistent AMPA Receptor Stimulation Alters [Ca-2+]i Homeostasis in Cultures of Embryonic Dopaminergic Neurons.  Mol. Brain Research 21, 303-311 1994.

Ravindranath, N., Papadopoulos, V., Brooker, G. and Dym, M.  Sertoli Cell Calcium Response to Basement Membrane and Follicle Stimulating Hormone.  Biology of Reproduction, 54, 130-137, 1996.

DeBernardi, M.A., Rabin, S., Colangelo, A. M , Brooker, G. and Mocchetti, I.  TrkA Mediates Nerve Growth Factor-Induced Intracellular Calcium Accumulation. J. Biol. Chem. 271, 6092-6098, 1996.

DeBernardi, M., Brooker, G. Single cell Ca-2+/cAMP crosstalk monitored by simultaneous Ca2+ cAMP fluorescence ratio imaging.  Proc. Natl. Acad. Sci. USA 93, 4577-4582, 1996.

DeBernardi, M.A., Brooker, G.  Simultaneous fluorescence ratio imaging of cyclic AMP and calcium kinetics in single living cells.  In Adenylate Cyclase (D. Cooper, editor), Advances in 2nd Messengers and Phosphoprotein Research. 32, 195-213, 1998.

Vanek, P. G, Somasundaram, B., Brooker, J. and Brooker, G.  Real-time, full spectrum confocal microscopy. American Biotechnology Laboratory, October, 2002.

Brooker, G.  Live web panel discussion on bio.com, In Focus, Examining Current Trends in Biotechnology -  Microscopy:  Applications in Cell Biology, January 30, 2003. http://www.bio.com/newsfeatures/newsfeatures_infocus.jhtml?InFocus=8832247

Joseph Rosen, Guy Indebetouw, and Gary Brooker  (2006). Homodyne scanning holography. Optics Express, Vol. 14, Issue 10, pp. 4280-4285.

DeBernardi M.A and Brooker, G. (2006). High content kinetic calcium imaging in drug sensitive and drug resistant human breast cancer cells. In Methods in Enzymology, Measurements of Biological Responses Using Automated Microscopy. Vol. 414, pp 317-335 (Editor: J. Inglese), Elsevier, San Diego, CA

Indebetouw G, Tada Y, Rosen J, and Brooker G. Scanning holography microscopy with resolution exceeding the Rayleigh limit of the objectives by superposition of off-axis holograms.  (2006) Applied Optics. Published on line Oct 16, 2006 doc. ID 74610

Joseph Rosen and Gary Brooker. Digital spatially incoherent Fresnel holography. Optics Letters 32, 912-914 (2007).

Joseph Rosen and Gary Brooker. Fluorescence Incoherent Color Holography. Optics Express 15, 2244-2250 (2007).

Joseph Rosen and Gary Brooker. Non-scanning motionless fluorescence three dimensional holographic microscopy. Nature Photonics 2, 190-195 (2008).

Joseph Rosen, Gary Brooker, Guy Indebetouw and Nathan T. Shaked. A Review of Incoherent Digital Fresnel Holography. Journal of Holography and Speckle 5, 124-140 (2009).

Joseph Rosen, Barak Katz and Gary Brooker. FINCH: Fresnel incoherent correlation hologram. Holography, Research and Technologies. J. Rosen, Ed. pp. 135-154 (Intech 2011).

Gary Brooker, Nisan Siegel, Victor Wang and Joseph Rosen. Optimal resolution in Fresnel incoherent correlation holographic fluorescence microscopy. Optics Express 19, 5047-5062 (2011)

Joseph Rosen, Nisan Siegel and Gary Brooker, Theoretical and experimental demonstration of resolution beyond the Rayleigh limit by FINCH fluorescence microscopic imaging. Opt. Exp. 19, 26249-26268 (2011).

 US Patents

3,959,077 Enzyme Preparation for Serum Cardiac Glycoside Assay.  Issued 5/25/1976.

4,022,577 Automated Radioimmunoassay.  Issued 5/10/1977.

4,104,026 Immunoassay Separation Technique. Issued 08/01/1978.

4,128,628 Automated Immunoassay.  Issued 12/05/1978.

4,376,125 Aminobenzylpropranolol and Pharmaceutical Preparation Thereof  Issued 03/08/1983.

5,332,905 Apparatus and Method for Multiple Emission Ratio Photometry and Multiple Emission Ratio Imaging.  Issued 07/26/1994.

5,491,343 High-Speed Multiple Wavelength Illumination Source, Apparatus Containing the Same, and Applications Thereof to Methods of Irradiating Luminescent Samples and of Quantitative Luminescence Ratio Microscopy.  Issued 02/13/1996.

5,723,943 Methods and Apparatuses for High-Speed Control of Lamp Intensities and/or Wavelengths for High Speed Optical Data Transmission  Issued 3/03/1998.

6,141,131 Methods and Apparatuses for High-Speed Control of Lamp Intensities and/or Wavelengths for High Speed Optical Data Transmission Issued 10/31/2000.

6,147,798 Method and Apparatus for Confocal Microscopy.  Issued 11/14/2000.

6,468,736 High Efficiency Cell Analysis and High Throughput Drug Screening System.  Issued 10/22/2002.

7,170,675 Method and System for Wide-Field Multi-Photon Microscopy Having a Confocal Excitation Plane. Issued 1/30/2007.

7,468,837 Wide-Field Multi-Photon Microscopy Having Simultaneous Confocal Imaging Over at Least Two Pixels. Issued 12/23/2008.


 
   
 
Home | Services | Fees | Gallery | Contacts | Sitemap
Copyright © 2011, Johns Hopkins University.
Optimized for 1024 x 768 resolution