Schedule and Readings for Schleif's Portion of Graduate Biophysical Chemistry, 2018
The following papers are available online through the Hopkins lilbrary. The book chapters are from "Genetics and Molecular Biology" by R. Schleif. You can download the
pdf of the entire book from this link.
1 Cell Structure, Feb. 15, Chap. 1
Inside a Living Cell, Goodsell, D., Trends in Biochemical
Sciences 16, 203-206 (1991).
2 Nucleic Acid Structure, Feb. 20, Chap. 2
DNA Flexibility Studied by Covalent Closure of Short
Fragments into Circles, D. Shore, J. Langoushi, R. Baldwin,
Proc. Natl. Acad. Sci. USA 78, 4833-4837 (1981).
3 DNA Synthesis, Feb.22, Chap. 3
E. coli oriC and
the dnaAGene Promoters are Sequestered from dam
Methyltransferase Following the Passage of the Chromosomal
Replication Fork, Campbell, J., Kleckner, N., Cell 62,
967-979 (1990).
4 RNA Synthesis, Feb. 27, Chap. 4
Transcription Against an Applied Force, H. Yin, M. Wang, K.
Svoboda, R. Landick, S. Block, J. Gelles, Science 270,
1653-1657 (1995).
5 RNA Synthesis, Mar.1
Promoter unwinding and promoter clearance by RNA polymerase:
Detection by single-molecule DNA nanomanipulation Andrey
Revyakin, Richard H. Ebright, and Terence R. Strick, Proc.
Nat. Acad. Sci. USA 101, 4778-80 (2004).
6 Small RNA's, Sept. 25 and 27. Chap. 5
Potent and Specific Genetic Interference by Double-stranded
RNA in Caenorhabditis elegans, A. Fire, S. Xu, M.
Montgomery, S. Kostas, S. Driver, C. Mello, Nature 391,
806-811 (1998).
7 Eukaryotic Nucleus, Oct. 2
Comprehensive Mapping of Long-Range Interactions Reveals
Folding Principles of the Human Genome, Erez
Lieberman-Aiden, Nynke L. van Berkum, Louise Williams, Maxim
Imakaev, Tobias Ragoczy, Agnes Telling, Ido Amit, Bryan R.
Lajoie, Peter J. Sabo, Michael O. Dorschner, Richard
Sandstrom, Bradley Bernstein, M. A. Bender, Mark Groudine,
Andreas Gnirke, John Stamatoyannopoulos, Leonid A. Mirny,
Eric S. Lander, and Job Dekker, Science 326: 289-293, (2009)
Exam, Oct. 4
8 Protein Structure, Mar. 29, Chap. 6
Xray Structure of the GCN4 Leucine Zipper, a Two-stranded,
Parallel Coiled Coil, E. O'Shea, J. Klemm, P. Kim, T. Alber,
Science 254, 539-544 (1991).
9 Protein Synthesis, April 3, Chap. 7
Role of a Peptide Tagging System in Degradation of Proteins
Synthesized from Damaged Messenger RNA, K. Keiler, P.
Waller, R. Sauer, Science 271, 990-993, (1996).
10 Genetics, Oct. 16, Chap. 8
General Model for Genetic Recombination, M. Meselson, C.
Radding, Proc. Natl. Acad. Sci. USA 72, 358-361 (1975).
11 Genetic Engineering, Oct. 18 Chaps. 9, 10
12 Genetic Engineering, Oct. 23
PCR Analysis of Tissue Samples from the 1979 Sverdlovsk
Anthrax Victims: The Presence of Multiple Bacillus Anthrax
Strains in Different Victims, P. Jackson, M. Hugh-Jones, D.
Adair, G. Green, K. Hill, C. Kuske, L. Grinberg, F.
Abromova, P. Keim, Proc. Natl. Acad. Sci. USA 95, 1224-1229
(1998).
13 Genetic Engineering, Oct. 25
An RNA Motif that Binds ATP, M. Sassanfar, J. Szostak,
Nature 364, 550-553 (1993).
14 Analysis of Sequence Similarity, Oct. 30
A General Method Applicable to the Search for Similarities
in the Anino Acid Sequence of two Proteins, S. Needleman, C.
Wunsch, J. Mol. Biol. 48, 443-53 (1970).
Exam, Nov. 1
15, 16 Ara and Lac Operons, Nov. 6, 8 Chaps. 11, 12
DNA Looping and Unlooping by AraC Protein, R. Lobell, R.
Schleif, Science 250, 528-532 (1990).
17 Lambda Phage, Nov. 13, Chap. 14
18 Lambda Phage, Nov. 15, Chap. 18
19 Transposable Elements, Nov. 27, Chap. 19
Genetic Evidence that Tn10 Transposes by a Nonreplicative
Mechanism, J. Bender, N. Kleckner, Cell 45, 801-815 (1986).
20 Molecular Aspects of the Immune System, Nov. 29,
Chap. 20
Cell-free V(D)J Recombination, D. Ramsden, T. Paull, M.
Gellert, Nature 388, 488-491 (1997).
21 Oncogenesis, Dec. 4 Chap. 23
Dietary Carcinogens and Anticarcinogens, B. Ames, Science
221, 1256-1264 (1983).
Exam, Dec. 6