Selected Recent Publications:

Urban S. and S. Moin. A class of membrane-altering agents and γ-secretase modulators provoke non-substrate cleavage by rhomboid proteases. (2014). Cell Reports, in press. 

Dickey S.W., Baker R.P., Cho S., and S. Urban. Proteolysis inside the membrane is a rate-governed reaction not driven by substrate affinity. (2013). Cell, 155(6): 1270-1281.
Link to Article

Moin S. and S. Urban. Membrane immersion allows rhomboid proteases to achieve specificity by reading transmembrane segment dynamics. (2012). eLife, 1: e00173. (DOI 10.7554/eLife.00173)
Link to Article

Baker R.P. and S. Urban. Architectural and thermodynamic principles underlying intramembrane protease function. (2012). Nature Chemical Biology 8(9): 759-768. (DOI 10.1038/nchembio.1021)
Link to Article

Zhou Y., Moin S.M., Urban S. and Y. Zhang. An internal water-retention site in the rhomboid intramembrane protease GlpG ensures catalytic efficiency. (2012) Structure 20(7): 1255-1263.
Link to Article

Parussini F., Tang Q., Moin S.M., Mital J., Urban S. and G.E. Ward. Intramembrane proteolysis of Toxoplasma aplical membrane antigen 1 facilitates host-cell invasion but is dispensable for replication. (2012). Proc. Natl. Acad. Sci. USA 109 (19): 7463-7468.
Link to Article

Urban, S. and Dickey S.W. The rhomboid protease family; a decade of progress on function and mechanism. (2011). Genome Biology, 12(10): 231-41.
Link to Article

Urban, S. Making the cut: central roles of intramembrane proteolysis in pathogenic microorganisms (2009). Nature Reviews Microbiology 7: 411-423 [cover article]
PubMed Abstract

Baxt, L.A., Baker R.P., Singh U., and Urban, S. An Entamoeba histolytica rhomboid protease with atypical specificity cleaves a surface lectin involved in phagocytosis and immune evasion. (2008). Genes & Development 22(12): 1636-1646. [cover article]
PubMed Abstract

Urban, S. and R.P. Baker. In vivo analysis reveals substrate-gating mutants of a rhomboid intramembrane protease display increased activity in living cells. (2008). Biological Chemistry 389: 1107-1115. [cover article]
PubMed Abstract

Urban, S. and Y. Shi. Core principles of intramembrane proteolysis: comparison of rhomboid and site-2 family proteases. (2008). Current Opinion in Structural Biology 18(4): 432-441.
PubMed Abstract

Baker, R.P., Young K., Feng L., Shi Y. and Urban, S. Enzymatic analysis of a rhomboid intramembrane proteases implicates transmembrane helix 5 as the lateral substrate gate. (2007). Proc. Natl. Acad. Sci. USA. 104 (20): 8257-8262. [cover article]
PubMed Abstract 

Wu Z., Yan N.,Feng L., Oberstein A., Yan H., Baker R. P., Gu L., Jeffrey P.D., Urban, S., and Y. Shi. Structural analysis of a rhomboid family intramembrane protease reveals a gating mechanism for substrate entry. (2006). Nature Structural and Molecular Biology. 13 (12): 1084-1091.
PubMed Abstract

Urban, S. Rhomboid proteins: conserved membrane proteases with divergent biological functions. (2006). Genes and Development. 20 (22): 3054-3068.
PubMed Abstract 

Baker, R.P., Wijetilaka R, and Urban, S. Two Plasmodium rhomboid proteases preferentially cleave different adhesins implicated in all invasive stages of malaria. (2006). PLoS Pathogens. 10(2): e113.
PubMed Abstract 

Brossier F., Jewett T., Sibley D. L., and Urban, S. A spatially-localized rhomboid protease cleaves cell surface adhesins essential for invasion by Toxoplasma. (2005). Proc. Natl. Acad. Sci. USA. <102(11):4146-4151.
PubMed Abstract

Urban, S. and M. S. Wolfe. Reconstitution of intramembrane proteolysis in vitro reveals that pure rhomboid is sufficient for catalysis and specificity. (2005). Proc. Natl. Acad. Sci. USA. 102(6):1883-1888.
PubMed Abstract

Urban, S. and M. Freeman. Substrate specificity of Rhomboid intramembrane proteases is governed by helix-breaking residues in the substrate transmembrane domain. (2003). Molecular Cell. 11: 1425-1434.
PubMed Abstract

Urban, S., Schlieper D., and M. Freeman. Conservation of intramembrane proteolytic activity and substrate specificity in prokaryotic and eukaryotic Rhomboids (2002). Current Biology. 12: 1507-1512.
PubMed Abstract

Urban, S., Lee J. R., and M. Freeman. A family of Rhomboid intramembrane proteases activates all Drosophila membrane-tethered EGF-like ligands (2002). EMBO Journal. 21: 4277-4286.
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Urban, S., Lee J. R., and M. Freeman. Drosophila Rhomboid-1 defines a family of putative intramembrane serine proteases. (2001). Cell. 107 (2): 173-182.
PubMed Abstract