Fleming Lab

Protocols

Protein Preps

• OMP Inclusion Body Prep
• Purifying OMPs on a Q column
• Purifying His-tagged proteins
• Purifying TEV
• TEV FAQs
• Using the Ultrafiltration Cell
• OMP amino acid sequences
• OMP nucleotide sequences

Instruments

• Using the French Press
• Using the FPLC

Lipids

• Drying Down Lipids
• Purifying Native E. coli Lipids
• Phosphate Assay
• Thin Layer Chromatography
• Sonicating SUVs

Miscellaneous

• Making Lab Stocks
• Making Urea
• Transformation by Electroporation
• Making Electrocompetent Cells